Meet an Early Career Ecologist: Christine Sit!

by Christine Sit

Hi! I’m Christine, I am a post-baccalaureate intern at Archbold Biological Station. I am able to be here because of a generous grant from the Vaughn-Jordan Foundation, who support research internships at Archbold’s Plant Ecology Program. I began my internship four months ago and am now in the middle of my project!

Title: Appreciating a Florida Scrub Jay. The image shows me in a black, long-sleeved shirt and ball cap with my left hand outstretched towards the right. A Florida Scrub Jay faces the camera while standing on my hand. Its mouth is open and has a peanut inside. To the background are pine trees and shorter scrub shrubs against a blue sky with a few clouds.

The author, Christine, appreciating a Florida Scrub Jay (which is appreciating a peanut)

My project focuses on plant functional traits in three different habitats of different elevations of the Florida scrub. From highest elevation to lowest, the habitats are Rosemary Balds, Scrubby Flatwoods, and Flatwoods.

Florida elevation does not change much. The lowest point at Archbold is about 40 meters above sea level, and the highest is only about 60 meters. However, these elevation differences are enough of a difference for the species that live in the Florida scrub.

The wet-dry season cycles and wildfires (or prescribed burns) interested me, so my initial project was to study the impact of soil moisture on plant functional traits to do with water intake, retention, and loss. Specific leaf area would indicate evapotranspiration from the higher or lower surface area; stomatal density would indicate water loss through gas exchange; root density at different depths in a given volume of soil would indicate where the roots wanted to be to take in the most water; and leaf water potential would indicate plant water stress.

Well…that idea lasted about two months.

My water idea mostly hinged on the one trait tying everything together, leaf water potential. I would use the lab’s Scholander Pressure Chamber, last used fifteen years ago. Then, after multiple days of cleaning and checking different things, we put the Chamber to the test. We found one leak. Then another. And another.

 

At this point, we will try one more time, but I have also adjusted my project’s focus to continue without this trait. Fortunately, plant functional traits haven’t been studied much in the area, so there is much to explore.

Title: The Multi-Decade-Old, Not-Quite-Functioning, 3-Legged Pressure Chamber The image shows a metal contraption called a Scholander Pressure Chamber. It sits on a wooden floor in front of a table and rolling chair. The Chamber has a nitrogen gas tank strapped to the bottom, a small pressure meter to the left of the gas tank, a larger pressure meter on the top, a metal cylinder to the upper left, and two valves to the right of the pressure meter. It is missing its front right leg, so it only stands upright with the support of my finger.

The Multi-Decade-Old, Not-Quite-Functioning, 3-Legged Pressure Chamber

Perhaps unsurprisingly to experienced researchers, my project’s focus has shifted since the beginning. I’ve debated which root traits to study for a while, but I’m settling on a couple. My root aspect worried me from the beginning because of how intensive the sampling is. However, another project in the lab began root sampling for mycorrhizae. By joining the sampling, I understood precisely how intensive the sampling would be.

Fortunately, the other project is looking at the tiniest, fine roots for mycorrhizae. In addition, the species match a few of mine, so I can actually use the larger primary and secondary roots for my project. In this way, I already have collected specimens!

Across my three habitats, I have seven shrubby plants. Four species are from the Ericacea family (Lyonia lucida, Lyonia fruticosa, Bejaria racemosa, Ceratiola ericoides), and three are from the Fagacea family (Quercus inopina, Quercus geminata, Quercus chapmanii).

My traits also cover various parts of plants: leaves, stems, and roots. I am looking at the dry matter content of the different parts for its relation to flammability and lifespan. I will also look at stomatal density, which relates to the gas exchange of water, oxygen, and carbon dioxide. Given the wet-dry season influence, I’m particularly interested in these traits’ water and flammability aspects, even if my project has a broader focus than just water.

In many ways, this feels like my first real independent research project. That’s not to say that my undergraduate research experiences weren’t real, but they were much shorter-term and less involved on my end. This is the first time I am the sole person actively working and spending so much time on a project.

Naturally, I’ve spent so much more time on this research than on any other research project, just by the vast majority of my job being research-oriented. I’ve read so many more papers and looked more deeply into the methodology than for any other project. No one else knows more about what I’m doing than me.

I’ve learned so much about how research works in general as well. I’ve learned how much collaboration can go into a project, especially from other related, concurrent projects like the root mycorrhizae project.

What has also been interesting is the difference between my project’s focus and the Plant Lab’s focus. The Plant Lab focuses primarily on rare and endangered species, whereas my project looks at the most common species. The Plant Lab mainly monitors long-term studies, while mine looks at a snapshot of the current year and season.

During my time at Archbold, I’ve learned about how projects develop. My project has adjusted in the short term. The Plant Lab’s longer-term studies have also been adapting. For example, we recently changed one project from individual-level demography to species-level. Learning about the process for research projects has been really interesting.

I’m excited to see how my project develops as I get closer to the end of my internship. I’m looking forward to the next stage of sampling and processing, and I’m most excited to see what stories my project will tell in the end.

Left Image Title: Demography Study of Dicerandra thinicola, also known as Titusville mint The image shows sandy ground with fallen leaves on the left. I am kneeling on the fallen leaves with hands slightly outstretched while also making a funny face and tilting my head to the right. To my right are 30-40 pink flags, each one marking a new Dicerandra sprout.

Demography Study of Dicerandra thinicola

Image Title: Demography of Crotalaria avonensis, also known as Avon Park Harebells The image also shows me in a turquoise long-sleeve shirt and long brown pants. I’m sitting on mostly bare white sand, gesturing to blue flags to my left. Each flag marks an individual Crotalaria plant. I’m smiling while sitting on mostly bare sand that is dotted with some short plants. The background shows a few taller pine trees against a blue sky.

Demography study of Crotalaria avonensis